• Users Online: 64
  • Print this page
  • Email this page


 
 
Table of Contents
ORIGINAL ARTICLE
Year : 2020  |  Volume : 19  |  Issue : 1  |  Page : 20-24

Glycemic status in patients with primary hypothyroidism and its relation to disease severity


1 Department of Internal Medicine, Al-Yarmouk Teaching Hospital, Baghdad, Iraq
2 Specialized Centre for Endocrinology and Diabetes, Al-Kindy Hospital, Baghdad, Iraq
3 Department of Internal Medicine, College of Medicine, University of Babylon, Babil, Iraq

Date of Submission10-Mar-2020
Date of Acceptance10-Mar-2020
Date of Web Publication20-Aug-2020

Correspondence Address:
Dr. Atheer Yass Ali
Department of Internal Medicine, Al-Yarmouk Teaching Hospital, Baghdad
Iraq
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/MJ.MJ_5_20

Get Permissions

  Abstract 


Background: Primary hypothyroidism can be defined as an increase in serum thyroid-stimulating hormone (TSH) level, and the concentration of free T3, free T4, T3, and T4 is low. Hypothyroidism is a prevalent disease mostly affecting middle-aged women. One of the most important determinants of glucose homeostasis is thyroid hormones. Hypothyroid patients have a higher prevalence of insulin resistance and tendency to Type 2 diabetes mellitus than the normal individual. Objective: To investigate the correlation between TSH and hemoglobin A1c (HbA1C) in patients with primary hypothyroidism; so, to study the effects of hypothyroidism on glucose metabolism. Subjects and Methods: Ninety-five Iraqi primary hypothyroidism patients and 40 healthy persons taken as control were selected from Specialized Centre for Endocrinology and Diabetes during the period from June 2017 to January 2018. The patients were diagnosed previously as cases of primary hypothyroidism, and they were on treatment and still on treatment. All patients were sent to investigate TSH, T4, T3, and HbA1c. The patients were subdivided into three main groups: first is uncontrolled nondiabetic primary hypothyroid group (36 patients), second is controlled nondiabetic primary hypothyroid group (43) and third is the diabetic primary hypothyroid group (16 patients). Results: A significant difference (P < 0.05) between HbA1C% in both controlled and uncontrolled hypothyroid groups against the control group, but there is no significant difference (P = 0.08) between the controlled and uncontrolled hypothyroid groups. The TSH relation with HbA1c was found to be significantly positive in the uncontrolled hypothyroid and diabetic, hypothyroid groups (r = 0.401 and 0.58, respectively). Conclusions: Significant increment was found in the level of HbA1c in hypothyroid patients, whether it is controlled or uncontrolled and a positive relationship was observed between TSH and HbA1c% in the uncontrolled and diabetic hypothyroid groups. Diabetes augments the effects of hypothyroidism on HbA1c.

Keywords: Glycemic status, insulin resistance, primary hypothyroidism


How to cite this article:
Ali AY, Allehibi KI, Al-Juboori NA. Glycemic status in patients with primary hypothyroidism and its relation to disease severity. Mustansiriya Med J 2020;19:20-4

How to cite this URL:
Ali AY, Allehibi KI, Al-Juboori NA. Glycemic status in patients with primary hypothyroidism and its relation to disease severity. Mustansiriya Med J [serial online] 2020 [cited 2020 Oct 27];19:20-4. Available from: https://www.mmjonweb.org/text.asp?2020/19/1/20/292714




  Introduction Top


Primary hypothyroidism

The central feature of hypothyroidism is the reduced production of thyroid hormone.[1],[2] Its prevalence increases with age, and the disease in women is about ten times more common than men. Hypothyroidism is more common in areas where there is iodine deficiency. Individuals who are at increased risk for developing hypothyroidism are those who have positive thyroid peroxidase antibodies and who have thyroid-stimulating hormone (TSH) values in the upper normal range.[3] Hypothyroidism affects carbohydrate metabolism in different ways:

  • First: It has been found that the thyroid hormone stimulates expression of the insulin-sensitive glucose transporter (GLUT4), so in hypothyroidism the levels of this transporter are usually low
  • Second: The activity of prohormone processing enzymes are increased in hypothyroidism, and this happened because one of important function of thyroid hormone, is downregulation of expression of these enzymes and this will lead to the slowing of degradation of insulin and increasing sensitivity to exogenous insulin. Hence, when hypothyroidism happened in patients with preexisting diabetes, there is a decrease in insulin requirement
  • Third: Hypothyroidism may affect glucose uptake at the tissue level. Local T3 production may be affected by polymorphisms in the 5-deiodinase type 2 (D2) genes, which may occur in hypothyroidism and decrease glucose uptake by tissues.[4]


The general changes in hypothyroidism will be reduced absorption of glucose from the gastrointestinal system, accumulation of glucose in peripheral tissues, the process of gluconeogenesis will be slowed, the output of glucose from the liver will diminish and increase tissue resistance to insulin entrance (i.e., increase insulin resistance).[5] The relationship between diabetes mellitus (DM) and thyroid dysfunction was found to be very strong.[6] Hypothyroidism (Hashimoto's thyroiditis) or thyroid hyperactivity (Graves' disease) has been investigated to be associated with DM. The frequency of thyroid dysfunction in the patients of DM about 11%.[7] It was found that thyroid disorders are increasing with age, and this happened globally, and all over the world, thyroid disease are less common in men than women. Subclinical hypothyroidism is reported to occur in about 4%–8.5% and may be reach 20% in females older than 60 years, while subclinical hyperthyroidism occurs less frequently and is reported to be about 2%.[8] In hypothyroidism, insulin resistance is proved and curtained in various in vitro and preclinical studies.[9],[10],[11]

It was found that the responsiveness of peripheral muscles to insulin action in hypothyroid patients became less. The dysregulation of metabolism of leptin could be responsible for such pathology.[11]

Many authors are document a direct relationship between insulin resistance and hypothyroidism.[12],[13],[14],[15] The insulin resistance not just associated with overt hypothyroidism but also with subclinical hypothyroidism[14],[16],[17]


  Subjects, Materials and Methods Top


Our study is a cross-sectional study and was done among 95 patients of diagnosed hypothyroidism from The Specialized Centre for Endocrinology and Diabetes and 40 persons who tested negative for thyroid disorder and they are not using any type of medication, with no mental or physical abnormality and no chronic disease was diagnosed previously, were taken as control for the study between the months June 2017 and January 2018, with an age range between 18 and 65 years. The patients were diagnosed previously based on the level of T3, T4 and TSH and they were on treatment and they were still on it during the study period.

Inclusion criteria

  1. Patient who already diagnosed previously as a case of primary hypothyroidism and on thyroxin treatment
  2. Patients who accept participation.


Exclusion criteria

  1. History of pituitary or hypothalamic disease or surgery
  2. Renal failure or dialysis
  3. Pregnant hypothyroid patient
  4. Any haemoglobinopathy
  5. Hemolytic disorder
  6. Recent (<3 months duration) blood transfusion.


All patients were sent for the following investigations:

1 – TSH 2 – Hemoglobin A1c (HbA1c%): We used the GX assay Kit, which used exclusively with the Tosoh Automated Glycohemoglobin Analyzer HLC-723 GX referred to as HLC-723GX in the IFU), which is based on the principle of high-performance liquid chromatography assay.

Statistical analysis

Results were presented as mean ± standard deviation for quantitative variables, and it was done by Statistical analysis were performed using MedCalc for windows, version 15.0 (MedCalc Software, Ostend, Belgium) and by the same program P value was calculated:

P < 0.05 → significant P > 0.05 → not significant.

Filtration of the results and figures was done by the excel program.

Correlation of the results (Pearson's correlation coefficient) was done by IBM Corp. Released 2015. IBM SPSS Statistics for windows, version 23.0 Armonk, NY: IBM Corp.

The patients were subdivided into three main groups according to the controlling of thyroid status based on TSH results and according to the presence of diabetes:

  • First group: Uncontrolled, not diabetic overt hypothyroidism this group comprised (36 patients) (TSH between 5.6 and 53.3 mU/L)
  • Second group: Controlled not diabetic hypothyroidism (43 patients) (TSH between 0. 5 and 4.5 mU/L)
  • Third group: Diabetic hypothyroidism (16 patients).



  Results Top


HbA1c levels in uncontrolled overt hypothyroid group were found to be in a range of 4.8%–6.3% with a mean of 5.78 ± 0.806 against the range of 4.8%–6.2% with the mean of 5.28 ± 0.45 in the healthy control group. The difference was found to be highly significant (P = 0.0012), while HbA1c in the uncontrolled hypothyroid group is not statistically different against HbA1c in controlled hypothyroid group were in the range of 4.9%–6.3% with the mean of 5.532 ± 0.452 (P = 0.082). All these findings are summarized in [Table 1].
Table 1: The comparison of age, thyroid-stimulating hormone, hemoglobin A1c in the uncontrolled overt hypothyroidism group with control group and also with controlled hypothyroidism group

Click here to view


HbA1c level in controlled hypothyroid group were found to be in a range of 4.9%–6.3% with a mean of 5.532 ± 0.452 against the range of 4.8%–6.2% with the mean of 5.28 ± 0.45 in the healthy control group and the difference was found to be significant (P = 0.014) and at the same time HbA1c in the diabetic, hypothyroid group were found to be in a range of 6%–13.7% with a mean of 9.262, and it is higher in that of controlled hypothyroid patients and significantly different (P < 0.0001).

These findings are summarized in [Table 2].
Table 2: The comparison of age, thyroid-stimulating hormone, and hemoglobin A1c in the controlled hypothyroidism group with healthy control group and also with diabetic hypothyroid group

Click here to view


When we studied the correlation between TSH and HbA1c, it was found to be a weak positive correlation in the healthy control group and controlled hypothyroid group. However, there is a significant positive linear correlation between TSH and HbA1c in the uncontrolled nondiabetic hypothyroid group (r = 0.401, strength of correlation: medium) and also in the diabetic hypothyroid group (r = 0.585, strength of correlation: strong).

These relations are shown in [Table 3] and [Figure 1], [Figure 2], respectively.
Table 3: Comparison and correlation between thyroid-stimulating hormone and hemoglobin A1c (%) in different study groups

Click here to view
Figure 1: Correlation between thyroid-stimulating hormone and hemoglobin A1c percentage in nondiabetic uncontrolled hypothyroidism group (r = 0.401)

Click here to view
Figure 2: Thyroid-stimulating hormone and hemoglobin A1c percentage correlation in diabetic hypothyroidism group (r = 0.585)

Click here to view



  Discussion Top


The results of the present study showed that by comparison between HbA1c% for both uncontrolled and controlled hypothyroid nondiabetic groups against A1c in the healthy control group were found to be that it is higher with a significant difference (P = 0.001 and 0.01 respectively) when compared with the control group [Table 1] and [Table 2].

The result of the relationship between the level of TSH and the HbA1c% showed that there is a significant positive correlation in the uncontrolled hypothyroid group (r = 0.401 level of strength: medium) [Table 3] and [Figure 1]. The TSH and A1C correlation in the diabetic hypothyroid group also positive, r = 0.58 level of strength: strong) [Table 3] and [Figure 2]. These relations were found to be weak in the controlled hypothyroid group.

These results indicate that thyroid hormones act on glucose homeostasis as an important mediator although the mechanism that causes this increase in HbA1c in hypothyroid patients is still unclear.[18]

Many theories were put on to explain the dysglycemia that happens in hypothyroidism:

  1. Thyroid hormones are important for glucose homeostasis and for glucose absorption, so in hypothyroidism decrease absorption leads to decrease utilization and this leads to increase in insulin level and increase resistance to insulin that will contribute to the elevation of blood glucose[19]
  2. Insulin resistance is the cause of blood glucose elevation in hypothyroidism, the accepted explanation for insulin resistance mechanism is that insulin hormones work synergically with thyroid hormone to exert its action such as up regulation of genes such as GLUT4 that used in glucose transport and phosphor glycerate kinase that involved in the process of glycolysis. Therefore, in hypothyroidism, the peripheral tissue showed resistance to insulin action[20],[21]
  3. Thyroid hormones have an important role at the cellular level by increasing mitochondrial biogenesis, activation of Tricarboxylic acid cycle (TCA) cycle and oxidation of fatty acid.


  4. In Type 2 diabetes, clearly demonstrated that the role of mitochondrial dysfunction leading to the accumulation of cellular lipid and oxidative metabolism impairments. Also, in skeletal muscle, it was found that the lack of thyroid hormones may lead to dysregulation of mitochondrial gene expression.

    The transcriptional regulator of mitochondrial content and function is the PPAR gamma coactivator-1alpha (PGC-1alpha) and this regulator plays an essential role in the oxidation of fatty acid and in gluconeogenesis.

    This PGC-1 that involved in the regulation of mitochondrial function requires thyroid hormone for its action. When give T3 treatment, PGC-1alpha gene expression increased by as much as 13 folds 6 h posttreatment, so in hypothyroidism decrease in the level of T3 lead to PGC1 alpha dysregulation and this lead to insulin resistance.[22]

  5. Hypothyroidism leads to decrease basal metabolic rate, so this leads to decrease in protein turnover that's mean prolongation of half-life
  6. Hypothyroidism is a state of increased oxidative stress that may lead to increased protein glycation.[19]


In this study, any of these theories can be applied as a cause of increase HbA1c and could be there is another factor that is responsible so difficult to differentiate the causative agent of increment A1c.

These relations had been demonstrated by another studies like a study of 45 hypothyroid patients in which HbA1c was higher than that of control (5.54 ± 0.43% vs. 5.34 ± 0.31% in hypothyroid patients and control respectively P < 0.001).[23]

Another study done by (Billic-Komarica et al. 2012) demonstrated the positive relationship between the level of TSH and HbA1c%.

In our study, the difference in HbA1c% in the uncontrolled and controlled hypothyroid group were found to be not significant (P = 0.08) [Table 1] and as it was mentioned both groups were found to be significantly different from the healthy control group (P = 0.001 and 0.01 respectively), so this raises a question, why controlling of hypothyroidism by thyroxin does not reversing the increment in HbA1c to the level of healthy control group? Is the insulin resistance could be responsible for this increase in HbA1c is irreversible?

The correct answer was not clear, but this may be the changes that occur at the cellular level that lead to great damage that even with correction not returning to the normal state. Another possible effect is the suggestion that hypothyroidism could be falsely increasing the HbA1c levels as a study in India suggest.[24] This subject needs further studies for full explanation.


  Conclusions Top


A significant increment was found in the level of HbA1c in hypothyroid patients, whether its controlled or noncontrolled and a positive relationship was observed between TSH and HbA1c% in the uncontrolled group.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.



 
  References Top

1.
Vaidya B, Pearce SH. Management of hypothyroidism in adults. BMJ 2008;337:a801.  Back to cited text no. 1
    
2.
Almandoz JP, Gharib H. Hypothyroidism: Etiology, diagnosis, and management. Med Clin North Am 2012;96:203-21.  Back to cited text no. 2
    
3.
Devdhar M. Ousman YH, Burman KD. Hypothyroidism. Endocrinology and metabolism clinics of North America 2007;36:595-615.  Back to cited text no. 3
    
4.
Chidakel A, Mentuccia D, Celi FS. Peripheral metabolism of thyroid hormone and glucose homeostasis. Thyroid 2005;15:899-903.  Back to cited text no. 4
    
5.
Althausen TL, Stockholm M. The influence of the thyroid gland on absorption in the digestive tract. Am J Physiol 1938;123:577-88.  Back to cited text no. 5
    
6.
Brenta G, Danzi S, Klein I. Potential therapeutic applications of thyroid hormone analogs. Nat Clin Pract Endocrinol Metab 2007;3:632-40.  Back to cited text no. 6
    
7.
Kadiyala R, Peter R, Okosieme OE. Thyroid dysfunction in patients with diabetes: Clinical implications and screening strategies. Int J Clin Pract 2010;64:1130-9.  Back to cited text no. 7
    
8.
Donckier JE. “Endocrine disease and diabetes” in Text Book of Diabetes Mellitus, J.C. Pickup and G. Williams, Eds., vol.27, Blackwell Publishing Company, Chichester, UK, 2003. p. 21-7.  Back to cited text no. 8
    
9.
Dimitriadis G, Parry-Billings M, Bevan S, Leighton B, Krause U, Piva T, et al. The effects of insulin on transport and metabolism of glucose in skeletal muscle from hyperthyroid and hypothyroid rats. Eur J Clin Invest 1997;27:475-83.  Back to cited text no. 9
    
10.
Dubaniewicz A, Kaciuba-Usciłko H, Nazar K, Budohoski L. Sensitivity of the soleus muscle to insulin in resting and exercising rats with experimental hypo-and hyper-thyroidism. Biochem J 1989;263:243-7.  Back to cited text no. 10
    
11.
Cettour-Rose P, Theander-Carrillo C, Asensio C, Klein M, Visser TJ, Burger AG, et al. Hypothyroidism in rats decreases peripheral glucose utilisation, a defect partially corrected by central leptin infusion. Diabetologia 2005;48:624-33.  Back to cited text no. 11
    
12.
Stanická S, Vondra K, Pelikánová T, Vlcek P, Hill M, Zamrazil V. Insulin sensitivity and counter-regulatory hormones in hypothyroidism and during thyroid hormone replacement therapy. Clin Chem Lab Med 2005;43:715-20.  Back to cited text no. 12
    
13.
Rochon C, Tauveron I, Dejax C, Benoit P, Capitan P, Fabricio A, et al. Response of glucose disposal to hyperinsulinaemia in human hypothyroidism and hyperthyroidism. Clin Sci (Lond) 2003;104:7-15.  Back to cited text no. 13
    
14.
Handisurya A, Pacini G, Tura A, Gessl A, Kautzky-Willer A. Effects of T4 replacement therapy on glucose metabolism in subjects with subclinical (SH) and overt hypothyroidism (OH). Clin Endocrinol (Oxf) 2008;69:963-9.  Back to cited text no. 14
    
15.
Dimitriadis G, Mitrou P, Lambadiari V, Boutati E, Maratou E, Panagiotakos DB, et al. Insulin action in adipose tissue and muscle in hypothyroidism. J Clin Endocrinol Metab 2006;91:4930-7.  Back to cited text no. 15
    
16.
Maratou E, Hadjidakis DJ, Peppa M, Alevizaki M, Tsegka K, Lambadiari V, et al. Studies of insulin resistance in patients with clinical and subclinical hyperthyroidism. Eur J Endocrinol 2010;163:625-30.  Back to cited text no. 16
    
17.
Dessein PH, Joffe BI, Stanwix AE. Subclinical hypothyroidism is associated with insulin resistance in rheumatoid arthritis. Thyroid 2004;14:443-6.  Back to cited text no. 17
    
18.
Boelen A. Thyroid hormones and glucose metabolism: The story begins before birth. Exp Physiol 2009;94:1050-1.  Back to cited text no. 18
    
19.
Soni N, Kaushik GG, Yadav V. TO study glycemic status and lipid fractions in subclinical hypothyroid, overt hypothyroid and hyperthyroid subjects. Int J Adv Res 2014;2:761-7.  Back to cited text no. 19
    
20.
Weinstein SP, O'Boyle E, Haber RS. Thyroid hormone increases basal and insulin-stimulated glucose transport in skeletal muscle. The role of GLUT4 glucose transporter expression. Diabetes 1994;43:1185-9.  Back to cited text no. 20
    
21.
Moeller LC, Dumitrescu AM, Walker RL, Meltzer PS, Refetoff S. Thyroid hormone responsive genes in cultured human fibroblasts. J Clin Endocrinol Metab 2005;90:936-43.  Back to cited text no. 21
    
22.
Brenta G. Why can insulin resistance be a natural consequence of thyroid dysfunction? J Thyroid Res 2011;2011:Article ID 152850, 9 pages, 2011.  Back to cited text no. 22
    
23.
Kim MK, Kwon HS, Baek KH, Lee JH, Park WC, Sohn HS, et al. Effects of thyroid hormone on A1C and glycated albumin levels in nondiabetic subjects with overt hypothyroidism. Diabetes Care 2010;33:2546-8.  Back to cited text no. 23
    
24.
Anantarapu S, Vaikkakara S, Sachan A, Phaneendra BV, Suchitra MM, Reddy AP, et al. Effects of thyroid hormone replacement on glycated hemoglobin levels in non diabetic subjects with overt hypothyroidism. Arch Endocrinol Metab 2015;59:495-500.  Back to cited text no. 24
    


    Figures

  [Figure 1], [Figure 2]
 
 
    Tables

  [Table 1], [Table 2], [Table 3]



 

Top
 
  Search
 
    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
    Access Statistics
    Email Alert *
    Add to My List *
* Registration required (free)  

 
  In this article
Abstract
Introduction
Subjects, Materi...
Results
Discussion
Conclusions
References
Article Figures
Article Tables

 Article Access Statistics
    Viewed375    
    Printed23    
    Emailed0    
    PDF Downloaded36    
    Comments [Add]    

Recommend this journal