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Year : 2022  |  Volume : 21  |  Issue : 1  |  Page : 41-47

Hepatoprotective effects of alpha-lipoic acid, Vitamin C alone, or in combination on methotrexate-induced liver injury

Department of Pharmacology, Al-mustansiriya University, Collage of Medicine, Baghdad, Iraq

Correspondence Address:
Dr. Raghad Raheem Abd Hasan
Department of Pharmacology, College of Medicine, Al-Mustansiriyah University, Baghdad
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/mj.mj_23_21

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Background: Different mechanisms have been attributed to methotrexate (MTX)-induced liver injury, one of which is related to MTX generation of free radicals and inducing the disturbance of oxidative stress biomarker. Alpha-lipoic acid (ALA) is a strong antioxidant dietary supplement and has an anti-inflammatory effect. Vitamin C is an antioxidant and might produce an anti-inflammatory action through its antioxidant effect. The Aim of the Current Study: To evaluate the effect of ALA and Vitamin C single or concurrent use of them on MTX-induced liver injury. Materials and Methods: Thirty-five adult male albino mice were divided into five groups: Group 1 were administered distilled water and sodium bicarbonate orally by oral gavage for 10 days and injected normal saline intraperitoneally (ip) in the 10th day, Group 2 were injected MTX ip on the 10th day only, Group 3 were administered 100 mg/kg Vitamin C orally for 10 days and injected MTX ip on the 10th day, Group 4 were administered ALA 60 mg/kg orally for 10 days and injected MTX ip on the 10th day, Group 5 were administered both ALA and Vitamin C orally and injected MTX ip on the 10th day. After 2 days of the last treatment, the animal was anesthesized and the blood was withdrawn to be used for biochemical assessment of liver functions and liver tissue was dissected out to be used for oxidative stress biomarker determination and histopathological study. Results: MTX group showed an increase in serum level of alanine aminotransferase, alkaline phosphatase, and lactate dehydrogenase, an increase liver tissue malondialdehyde (MDA), and a reduction of tissue glutathione and superoxide dismutase (SOD), while the treatment groups showed an improvement of liver enzyme elevation and decreased lipid peroxidation MDA and increased SOD. Conclusion: Pretreatment by using ALA alone or vitamin alone provides comparable improvement of liver histology and liver injury while combined regimen did not provide more significant improvement of liver injury than using each one alone.

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